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human igg4 pfc pe southern biotech  (SouthernBiotech)


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    Structured Review

    SouthernBiotech human igg4 pfc pe southern biotech
    Human Igg4 Pfc Pe Southern Biotech, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+igg4+pfc+pe+southern+biotech/Mouse+Anti-Human+IgG4+pFc'-PE/pm24053626__cb4004942_si_001-454-225-230
    Average 93 stars, based on 8 article reviews
    human igg4 pfc pe southern biotech - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Modification:

    Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators
    Article Snippet: Equipment, Materials, and Software •C6 Flow Cytometer Accuri with CFlow Plus software •Eppendorf tubes sterilized in autoclave before use •FlowJo software •Graphpad Prism software (version 5.0b for Mac) •T-Flasks BD Falcon #353136 75 cm2 tissue culture treated Buffers and Solutions •pH were adjusted with 1 M NaOH or 2 M HCl. .. •DPBS Solution Invitrogen #14190-144 •Dulbecco’s modified Eagle’s medium ATCC #30-2002 •EDTA Detachment Solution 210 mL DPBS 392 mg EDTA disodium salt (5.0 mM) 84 mg EGTA (1.0 mM) to pH 7.4 0.22 μM sterile filter •Flow Incubation Buffer 100 mL DPBS 12 mg NaN3 (2 mM) 180 mg EDTA (5 mM) to pH 7.4 100 mg BSA (1 mg / mL) stored at 4 oC •NHS Labeling Buffer DPBS to pH 8.5 0.22 μM sterile filter rechecked the pH weekly •Propidium Iodide Stock Solution 2.5 mg propidium iodide 7.5 mL DPBS = 500 μM stored at 4 oC in dark Antibodies, Reagents, and Pooled Serum •Albumin from Bovine Serum Sigma #A9647, Lot #050M1673V •Fetal Bovine Serum, Qualified, Heat-Inactivated Invitrogen #16140-071 The purchased solution split into aliquots, each of which was stored at -20 oC •Gt F(ab’)2 Anti Human IgG (γ) R-PE conj Invitrogen #H10104, Lot #1024854A stored at 4 oC •Gt F(ab’)2 Anti Human IgM (μ) R-PE conj Invitrogen #H15104, Lot #772646B stored at 4 oC •L-Rhamnose-BSA Dextra Laboratories #NGP1106, Lot #PCDX1182 •Mouse Anti Human IgG1 (hinge) - RPE (clone 4E3) Southern Biotech #9052-09 Lot #L110-NG11 stored at 4 oC •Mouse Anti Human IgG2 (Fc) - PE Southern Biotech #9060-09 Lot #J6810-XH80D stored at 4 oC •Mouse Anti Human IgG3 (hinge) - PE Southern Biotech #9210-09 Lot #A5711-Q291 stored at 4 oC S65 •Mouse Anti Human IgG4 (pFc’) - PE Southern Biotech #9190-09 Lot #F0810-VI50Z stored at 4 oC •Pooled Complement Preserved Human Serum Innovative Research #HC1004, Lot #F9100 The purchased solution split into aliquots, each of which was stored at -80 oC •Propidium Iodide Sigma #P4170, Lot #081M1254V •Trypan Blue Stain 0.4% Invitrogen #15250 A172 Cell Culture •All cell lines were grown in an incubator (37 oC) supplemented with 5% CO2. ..

    Sterility:

    Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators
    Article Snippet: Equipment, Materials, and Software •C6 Flow Cytometer Accuri with CFlow Plus software •Eppendorf tubes sterilized in autoclave before use •FlowJo software •Graphpad Prism software (version 5.0b for Mac) •T-Flasks BD Falcon #353136 75 cm2 tissue culture treated Buffers and Solutions •pH were adjusted with 1 M NaOH or 2 M HCl. .. •DPBS Solution Invitrogen #14190-144 •Dulbecco’s modified Eagle’s medium ATCC #30-2002 •EDTA Detachment Solution 210 mL DPBS 392 mg EDTA disodium salt (5.0 mM) 84 mg EGTA (1.0 mM) to pH 7.4 0.22 μM sterile filter •Flow Incubation Buffer 100 mL DPBS 12 mg NaN3 (2 mM) 180 mg EDTA (5 mM) to pH 7.4 100 mg BSA (1 mg / mL) stored at 4 oC •NHS Labeling Buffer DPBS to pH 8.5 0.22 μM sterile filter rechecked the pH weekly •Propidium Iodide Stock Solution 2.5 mg propidium iodide 7.5 mL DPBS = 500 μM stored at 4 oC in dark Antibodies, Reagents, and Pooled Serum •Albumin from Bovine Serum Sigma #A9647, Lot #050M1673V •Fetal Bovine Serum, Qualified, Heat-Inactivated Invitrogen #16140-071 The purchased solution split into aliquots, each of which was stored at -20 oC •Gt F(ab’)2 Anti Human IgG (γ) R-PE conj Invitrogen #H10104, Lot #1024854A stored at 4 oC •Gt F(ab’)2 Anti Human IgM (μ) R-PE conj Invitrogen #H15104, Lot #772646B stored at 4 oC •L-Rhamnose-BSA Dextra Laboratories #NGP1106, Lot #PCDX1182 •Mouse Anti Human IgG1 (hinge) - RPE (clone 4E3) Southern Biotech #9052-09 Lot #L110-NG11 stored at 4 oC •Mouse Anti Human IgG2 (Fc) - PE Southern Biotech #9060-09 Lot #J6810-XH80D stored at 4 oC •Mouse Anti Human IgG3 (hinge) - PE Southern Biotech #9210-09 Lot #A5711-Q291 stored at 4 oC S65 •Mouse Anti Human IgG4 (pFc’) - PE Southern Biotech #9190-09 Lot #F0810-VI50Z stored at 4 oC •Pooled Complement Preserved Human Serum Innovative Research #HC1004, Lot #F9100 The purchased solution split into aliquots, each of which was stored at -80 oC •Propidium Iodide Sigma #P4170, Lot #081M1254V •Trypan Blue Stain 0.4% Invitrogen #15250 A172 Cell Culture •All cell lines were grown in an incubator (37 oC) supplemented with 5% CO2. ..

    Incubation:

    Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators
    Article Snippet: Equipment, Materials, and Software •C6 Flow Cytometer Accuri with CFlow Plus software •Eppendorf tubes sterilized in autoclave before use •FlowJo software •Graphpad Prism software (version 5.0b for Mac) •T-Flasks BD Falcon #353136 75 cm2 tissue culture treated Buffers and Solutions •pH were adjusted with 1 M NaOH or 2 M HCl. .. •DPBS Solution Invitrogen #14190-144 •Dulbecco’s modified Eagle’s medium ATCC #30-2002 •EDTA Detachment Solution 210 mL DPBS 392 mg EDTA disodium salt (5.0 mM) 84 mg EGTA (1.0 mM) to pH 7.4 0.22 μM sterile filter •Flow Incubation Buffer 100 mL DPBS 12 mg NaN3 (2 mM) 180 mg EDTA (5 mM) to pH 7.4 100 mg BSA (1 mg / mL) stored at 4 oC •NHS Labeling Buffer DPBS to pH 8.5 0.22 μM sterile filter rechecked the pH weekly •Propidium Iodide Stock Solution 2.5 mg propidium iodide 7.5 mL DPBS = 500 μM stored at 4 oC in dark Antibodies, Reagents, and Pooled Serum •Albumin from Bovine Serum Sigma #A9647, Lot #050M1673V •Fetal Bovine Serum, Qualified, Heat-Inactivated Invitrogen #16140-071 The purchased solution split into aliquots, each of which was stored at -20 oC •Gt F(ab’)2 Anti Human IgG (γ) R-PE conj Invitrogen #H10104, Lot #1024854A stored at 4 oC •Gt F(ab’)2 Anti Human IgM (μ) R-PE conj Invitrogen #H15104, Lot #772646B stored at 4 oC •L-Rhamnose-BSA Dextra Laboratories #NGP1106, Lot #PCDX1182 •Mouse Anti Human IgG1 (hinge) - RPE (clone 4E3) Southern Biotech #9052-09 Lot #L110-NG11 stored at 4 oC •Mouse Anti Human IgG2 (Fc) - PE Southern Biotech #9060-09 Lot #J6810-XH80D stored at 4 oC •Mouse Anti Human IgG3 (hinge) - PE Southern Biotech #9210-09 Lot #A5711-Q291 stored at 4 oC S65 •Mouse Anti Human IgG4 (pFc’) - PE Southern Biotech #9190-09 Lot #F0810-VI50Z stored at 4 oC •Pooled Complement Preserved Human Serum Innovative Research #HC1004, Lot #F9100 The purchased solution split into aliquots, each of which was stored at -80 oC •Propidium Iodide Sigma #P4170, Lot #081M1254V •Trypan Blue Stain 0.4% Invitrogen #15250 A172 Cell Culture •All cell lines were grown in an incubator (37 oC) supplemented with 5% CO2. ..

    Labeling:

    Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators
    Article Snippet: Equipment, Materials, and Software •C6 Flow Cytometer Accuri with CFlow Plus software •Eppendorf tubes sterilized in autoclave before use •FlowJo software •Graphpad Prism software (version 5.0b for Mac) •T-Flasks BD Falcon #353136 75 cm2 tissue culture treated Buffers and Solutions •pH were adjusted with 1 M NaOH or 2 M HCl. .. •DPBS Solution Invitrogen #14190-144 •Dulbecco’s modified Eagle’s medium ATCC #30-2002 •EDTA Detachment Solution 210 mL DPBS 392 mg EDTA disodium salt (5.0 mM) 84 mg EGTA (1.0 mM) to pH 7.4 0.22 μM sterile filter •Flow Incubation Buffer 100 mL DPBS 12 mg NaN3 (2 mM) 180 mg EDTA (5 mM) to pH 7.4 100 mg BSA (1 mg / mL) stored at 4 oC •NHS Labeling Buffer DPBS to pH 8.5 0.22 μM sterile filter rechecked the pH weekly •Propidium Iodide Stock Solution 2.5 mg propidium iodide 7.5 mL DPBS = 500 μM stored at 4 oC in dark Antibodies, Reagents, and Pooled Serum •Albumin from Bovine Serum Sigma #A9647, Lot #050M1673V •Fetal Bovine Serum, Qualified, Heat-Inactivated Invitrogen #16140-071 The purchased solution split into aliquots, each of which was stored at -20 oC •Gt F(ab’)2 Anti Human IgG (γ) R-PE conj Invitrogen #H10104, Lot #1024854A stored at 4 oC •Gt F(ab’)2 Anti Human IgM (μ) R-PE conj Invitrogen #H15104, Lot #772646B stored at 4 oC •L-Rhamnose-BSA Dextra Laboratories #NGP1106, Lot #PCDX1182 •Mouse Anti Human IgG1 (hinge) - RPE (clone 4E3) Southern Biotech #9052-09 Lot #L110-NG11 stored at 4 oC •Mouse Anti Human IgG2 (Fc) - PE Southern Biotech #9060-09 Lot #J6810-XH80D stored at 4 oC •Mouse Anti Human IgG3 (hinge) - PE Southern Biotech #9210-09 Lot #A5711-Q291 stored at 4 oC S65 •Mouse Anti Human IgG4 (pFc’) - PE Southern Biotech #9190-09 Lot #F0810-VI50Z stored at 4 oC •Pooled Complement Preserved Human Serum Innovative Research #HC1004, Lot #F9100 The purchased solution split into aliquots, each of which was stored at -80 oC •Propidium Iodide Sigma #P4170, Lot #081M1254V •Trypan Blue Stain 0.4% Invitrogen #15250 A172 Cell Culture •All cell lines were grown in an incubator (37 oC) supplemented with 5% CO2. ..

    Staining:

    Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators
    Article Snippet: Equipment, Materials, and Software •C6 Flow Cytometer Accuri with CFlow Plus software •Eppendorf tubes sterilized in autoclave before use •FlowJo software •Graphpad Prism software (version 5.0b for Mac) •T-Flasks BD Falcon #353136 75 cm2 tissue culture treated Buffers and Solutions •pH were adjusted with 1 M NaOH or 2 M HCl. .. •DPBS Solution Invitrogen #14190-144 •Dulbecco’s modified Eagle’s medium ATCC #30-2002 •EDTA Detachment Solution 210 mL DPBS 392 mg EDTA disodium salt (5.0 mM) 84 mg EGTA (1.0 mM) to pH 7.4 0.22 μM sterile filter •Flow Incubation Buffer 100 mL DPBS 12 mg NaN3 (2 mM) 180 mg EDTA (5 mM) to pH 7.4 100 mg BSA (1 mg / mL) stored at 4 oC •NHS Labeling Buffer DPBS to pH 8.5 0.22 μM sterile filter rechecked the pH weekly •Propidium Iodide Stock Solution 2.5 mg propidium iodide 7.5 mL DPBS = 500 μM stored at 4 oC in dark Antibodies, Reagents, and Pooled Serum •Albumin from Bovine Serum Sigma #A9647, Lot #050M1673V •Fetal Bovine Serum, Qualified, Heat-Inactivated Invitrogen #16140-071 The purchased solution split into aliquots, each of which was stored at -20 oC •Gt F(ab’)2 Anti Human IgG (γ) R-PE conj Invitrogen #H10104, Lot #1024854A stored at 4 oC •Gt F(ab’)2 Anti Human IgM (μ) R-PE conj Invitrogen #H15104, Lot #772646B stored at 4 oC •L-Rhamnose-BSA Dextra Laboratories #NGP1106, Lot #PCDX1182 •Mouse Anti Human IgG1 (hinge) - RPE (clone 4E3) Southern Biotech #9052-09 Lot #L110-NG11 stored at 4 oC •Mouse Anti Human IgG2 (Fc) - PE Southern Biotech #9060-09 Lot #J6810-XH80D stored at 4 oC •Mouse Anti Human IgG3 (hinge) - PE Southern Biotech #9210-09 Lot #A5711-Q291 stored at 4 oC S65 •Mouse Anti Human IgG4 (pFc’) - PE Southern Biotech #9190-09 Lot #F0810-VI50Z stored at 4 oC •Pooled Complement Preserved Human Serum Innovative Research #HC1004, Lot #F9100 The purchased solution split into aliquots, each of which was stored at -80 oC •Propidium Iodide Sigma #P4170, Lot #081M1254V •Trypan Blue Stain 0.4% Invitrogen #15250 A172 Cell Culture •All cell lines were grown in an incubator (37 oC) supplemented with 5% CO2. ..

    Cell Culture:

    Article Title: Exploring binding and effector functions of natural human antibodies using synthetic immunomodulators
    Article Snippet: Equipment, Materials, and Software •C6 Flow Cytometer Accuri with CFlow Plus software •Eppendorf tubes sterilized in autoclave before use •FlowJo software •Graphpad Prism software (version 5.0b for Mac) •T-Flasks BD Falcon #353136 75 cm2 tissue culture treated Buffers and Solutions •pH were adjusted with 1 M NaOH or 2 M HCl. .. •DPBS Solution Invitrogen #14190-144 •Dulbecco’s modified Eagle’s medium ATCC #30-2002 •EDTA Detachment Solution 210 mL DPBS 392 mg EDTA disodium salt (5.0 mM) 84 mg EGTA (1.0 mM) to pH 7.4 0.22 μM sterile filter •Flow Incubation Buffer 100 mL DPBS 12 mg NaN3 (2 mM) 180 mg EDTA (5 mM) to pH 7.4 100 mg BSA (1 mg / mL) stored at 4 oC •NHS Labeling Buffer DPBS to pH 8.5 0.22 μM sterile filter rechecked the pH weekly •Propidium Iodide Stock Solution 2.5 mg propidium iodide 7.5 mL DPBS = 500 μM stored at 4 oC in dark Antibodies, Reagents, and Pooled Serum •Albumin from Bovine Serum Sigma #A9647, Lot #050M1673V •Fetal Bovine Serum, Qualified, Heat-Inactivated Invitrogen #16140-071 The purchased solution split into aliquots, each of which was stored at -20 oC •Gt F(ab’)2 Anti Human IgG (γ) R-PE conj Invitrogen #H10104, Lot #1024854A stored at 4 oC •Gt F(ab’)2 Anti Human IgM (μ) R-PE conj Invitrogen #H15104, Lot #772646B stored at 4 oC •L-Rhamnose-BSA Dextra Laboratories #NGP1106, Lot #PCDX1182 •Mouse Anti Human IgG1 (hinge) - RPE (clone 4E3) Southern Biotech #9052-09 Lot #L110-NG11 stored at 4 oC •Mouse Anti Human IgG2 (Fc) - PE Southern Biotech #9060-09 Lot #J6810-XH80D stored at 4 oC •Mouse Anti Human IgG3 (hinge) - PE Southern Biotech #9210-09 Lot #A5711-Q291 stored at 4 oC S65 •Mouse Anti Human IgG4 (pFc’) - PE Southern Biotech #9190-09 Lot #F0810-VI50Z stored at 4 oC •Pooled Complement Preserved Human Serum Innovative Research #HC1004, Lot #F9100 The purchased solution split into aliquots, each of which was stored at -80 oC •Propidium Iodide Sigma #P4170, Lot #081M1254V •Trypan Blue Stain 0.4% Invitrogen #15250 A172 Cell Culture •All cell lines were grown in an incubator (37 oC) supplemented with 5% CO2. ..



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    93
    SouthernBiotech human igg4 pfc pe southern biotech
    Human Igg4 Pfc Pe Southern Biotech, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+igg4+pfc+pe+southern+biotech/Mouse+Anti-Human+IgG4+pFc'-PE/pm24053626__cb4004942_si_001-454-225-230
    Average 93 stars, based on 1 article reviews
    human igg4 pfc pe southern biotech - by Bioz Stars, 2026-09
    93/100 stars
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